ethionamide eto  (Thermo Fisher)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Sirt1 coordinates the mitochondrial UPR and myocellular proteostasis to preserve muscle integrity during muscle atrophy in zebrafish

doi: 10.3389/fcell.2026.1761278

Figure Lengend Snippet: Inhibition of the mFAO sensitizes muscle to atrophic stress. (a) qRT-PCR analysis of zebrafish larvae treated with DEXA(S) and/or etomoxir (ETO) showing the expression of the atrophy genes fbxo32 and trim63a (DEXA(S) indicates a short-course DEXA treatment. * p < 0.05, t-test, two-tailed, error bar indicates s.d.). (b) Representative fluorescent images of LysoView-stained zebrafish larvae treated with DEXA(S) and/or ETO. Histogram showing quantification of fluorescent intensity (white rectangle indicates the area chosen for the fluorescence quantification. Scale bar represents 1 mm. * p < 0.05, one-way ANOVA and Tukey’s multiple comparison test; error bar indicates s.d.). (c) Representative confocal microscopy images of Alexa488-phalloidin-stained zebrafish larvae treated with DEXA(S) and/or ETO with or without the hormesis treatment (arrows indicate examples of detached muscle fibers). Boxplot shows quantification of larvae with 0, 1–2, 3–15, or >15 detached muscle fibers (* p < 0.05, Chi-square test. Scale bar represents 50 µM).

Article Snippet: To block Cpt1 activity, larval zebrafish at 4 dpf were treated with 10 μM etomoxir (ETO) (Selleckchem) with or without DEXA for 18 h–20 h. To suppress autophagy, 5 mM chloroquine (Sigma) was added to the larval zebrafish medium for 1-h incubation.

Techniques: Inhibition, Quantitative RT-PCR, Expressing, Two Tailed Test, Staining, Fluorescence, Comparison, Confocal Microscopy

Journal: eLife

Article Title: S100a4 + alveolar macrophages accelerate the progression of precancerous atypical adenomatous hyperplasia by promoting the angiogenic function regulated by fatty acid metabolism

doi: 10.7554/eLife.101731

Figure Lengend Snippet: ( A ) Determination of intracellular lipid droplet accumulation, mitochondrial membrane potential, and mitochondrial reactive oxygen species (ROS) by flow cytometry, n = 3. ( B ) Tube formation of human umbilical vein endothelial cells (HUVECs) after coculture with S100a4 -OE MH-S, quantified by total tube length and number of nodes, n = 5. Scale bar: 200 µm. ( C ) Western blotting of fatty acid metabolism-related CPT1A in S100a4 -OE MH-S. ( D ) Western blotting of ANXA2 and PLG in S100a4 -OE MH-S and cocultured HUVEC and MLE12. ( E ) CCK8 assay of cocultured MLE12 after treatment of ETO to S100a4 -OE MH-S, n = 6. ( F ) Wound healing assay of cocultured MLE12 after treatment of ETO to S100a4 -OE MH-S, n = 3. Scale bar: 200 µm. ( G ) Intracellular ROS production of cocultured MLE12 after treatment of ETO to S100a4 -OE MH-S, n = 3. ( H ) Tube formation of cocultured HUVECs after treatment of ETO to S100a4 -OE MH-S, n = 3. Scale bar: 200 µm. ( I ) Western blotting of fatty acid metabolism-related proteins (CPT1A and PPAR-γ), angiogenesis-related proteins (ANXA2, VEGF, TGF-β, HIF-1α, and MMP9), and M2 polarization markers (CD206 and ARG1) in S100a4 -OE MH-S after treatment of ETO. ( J ) Western blotting of angiogenesis-related proteins (ANXA2, VEGF, and HIF-1α) in cocultured MLE12 after treatment of ETO to S100a4 -OE MH-S. The results shown above represent three or more replicates. MFI: mean fluorescence intensity; ETO: etomoxir; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Figure 6—source data 1. PDF file containing original western blots for , indicating the relevant bands. Figure 6—source data 2. Original files for western blot analysis displayed in . Figure 6—source data 3. PDF file containing original western blots for , indicating the relevant bands. Figure 6—source data 4. Original files for western blot analysis displayed in . Figure 6—source data 5. PDF file containing original western blots for , indicating the relevant bands. Figure 6—source data 6. Original files for western blot analysis displayed in . Figure 6—source data 7. PDF file containing original western blots for , indicating the relevant bands. Figure 6—source data 8. Original files for western blot analysis displayed in .

Article Snippet: ETO (MedChem Express) was used for targeted inhibition of CPT1A in MH-S.

Techniques: Membrane, Flow Cytometry, Western Blot, CCK-8 Assay, Wound Healing Assay, Fluorescence